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0 <br />2 <br />METHODS <br />9 <br />Fish History <br />Razorback suckers (Xyrauchen texanus) used in all experiments were <br />from a single female spawned in 1974 at the Willow Beach National Fish <br />Hatchery (WBNFH). The progeny were reared in concrete outdoor raceways <br />where they were fed commercial trout feed. Approximately 100 of the <br />largest specimens (30-40 cm in length) were shipped to California in the <br />10 <br />0 <br />r <br />spring, 1980. Remaining fish (n = 700, 15-30 cm in length) were trans- <br />ported to the Utah Water Research Laboratory (UWRL), Logan, Utah on June <br />17, 1980. Fish were held in circular 833-liter tanks under controlled <br />photoperiod (14L x lOD) and acclimated to 8, 14, 20, 26 + 1°C. Fish <br />were fed Silver Cup trout chow (3 mm pellets) ad libitum once per day. <br />Terramycin was added to the feed (4-10 mg/1) for two 7-day periods to <br />control columnaris infection. Diseased fish were not subjected to testing <br />of any kind during treatment or for 7 days thereafter. <br />Juvenile Colorado squawfish (Ptychocheilus lucius) that were used <br />10 in temperature preference, blood analysis, and swimming stamina studies <br />were hatched on July 1, 1979, from hatchery-reared adults at the WBNFH. <br />The progeny were held in concrete outdoor raceways and fed commercial <br />10 trout feed. The fish were transported to the Fisheries Experiment Station <br />(FES), Logan, Utah in February, 1980, and then on July 17, 1980, they <br />were moved to the UWRL facility. At UWRL, they were held in circular <br />225-liter tanks fed Silver Cup commercial trout food (#3 crumbles) ad. <br />libitum twice per day, kept on a 14L x lOD photoperiod, and were <br />acclimated to 14, 20, 26 ± PC. The fish ranged in length from 7.5 to <br />10 cm (n = 1000) when received and appeared to grow best at 20°C (after <br />0