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45 residue half-life in Daphnia was 44 h; however, neither trout nor Daphnia appeared to degrade HCB sig- nificantly. Preliminary food chain studies indicated that bluegills accumulated HCB to a level 1 to 2 times that observed for DDT when they were fed Daphnia containing residues of 50 ng/g. In comparison, bluegills accumulated residues about half that of DDT in water exposure at concentrations of only 0.05 to 0.15,ug/L. After 5 days of incubation, both aerobically and anaerobically, hydrosoil samples inoculated with ring labeled HCB showed an 80 to 90% loss of mate- rial. Although phenolic volatilization may be responsible for some of this loss, degradation products were revealed, one of which has been tentatively iden- tified as pentachlorophenol. NOTE: Flow-through toxicity tests produced TILC50's ranging from 0.54 mg/L for rainbow trout to 6.60 mg/L for fathead minnows. Cumulative toxicity indices ranged only from 1.20 to 3.14, indicating little or no cumulative toxic action. Sublethal effects were prominent in flow-through tests at concentrations considerably below acutely lethal levels. Such effects included early cessation of feeding, hypersensitivity, and erratic swimming, followed by a loss of motor function, hemorrhage in the dorsal fin region, and occurrence of a characteristic "broken back" syn- drome. This condition remained for extended periods until terminated by death from secondary causes. HOUGHTO-SAFE 1120 KEPONE Chemical Name: Mixture of tri-aryl phosphate esters containing phenyl, tolyl (cresyl), xylyl, and ethyl Chemical Name: Decachlorooctahydro-1,3,4-metheno- substituted benzene groups 2H-cyclobuta [cd] pentalen-2-one Alternate Names: Tri-aryl phosphate ester Alternate Names: CAS 143-50-0, ENT-16391, GC- 1899,Chlordecone Principal Use: Hydraulic fluids Sample Description: Commercial formulations, 100% SUMMARY OF ACUTE TOXICITY 96-h LC50 Test Stage or Temp 95% CI organism wt (g) (C) (mg/L) G. pseudolimnaeus M 20 0.708 0.53-0.92 Coho salmon 0.4 12 1.2b 0.9-1.5 Rainbow trout 1.4 12 1.7 1.2-2.5 Brook trout 4.0 12 3.6b 3.1-4.2 Fathead minnow 1.2 17 35 24.49 Principal Use: Insecticide Sample Description: Technical material, 90.7% SUMMARY OF ACUTE TOXICITY 96-h LC50 Test Stage or Temp 95% CI organism wt (g) (C) (µg/L) Daphnia magna 11 17 2608 200-345 G. pseudolimnaeus M 17 180 110-290 Chironomus I4 22 320a 220-450 Rainbow trout 1.1 12 30 24-38 Channel catfish 1.6 18 225 Channel catfish 1.6 21 43 Bluegill 31-59 Bluegill 0.6 20 12 8-19 Yellow perch 7.2 12 0.54a.b 0.42-0.70 aTested in hard water. bFlow-through toxicity test. 2-5 24 72 Redear sunfish 1.0 24 44 41-47 848-h EC50. NOTE: Variation in water hardness from 44 to 272 ppm did not alter toxicity to rainbow trout. An in- crease in temperature from 7 ° to 29 °C produced a 4.8- fold increase in toxicity.