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<br />1 <br />f <br /> <br />1 <br />1 <br /> <br /> <br />1 <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br />The results provided by macroinvertebrate sampling efforts and accurate identifications <br />can provide a great deal of valuable information pertaining to the flow regime and other <br />aquatic conditions. <br />Sampling was conducted during spring and fall of 2001. Four sites were sampled in the <br />spring and five sites in the fall. At each location three samples were taken in riffle <br />habitat. All samples at a given site location were taken in areas of similar size substrate <br />to avoid bias that may be directly related to substrate size. Because of its effectiveness at <br />various flows, a Hess Sampler was used during sampling to provide quantitative <br />invertebrate data. Macroinvertebrates were preserved in ethanol and transported to the lab <br />where they were sorted, enumerated and identified to the lowest practical taxonomic level <br />(Merritt and Cummins 1984; Ward and Kondratieff 1992). The "lowest practical taxonomic <br />level" means that all specimens will be identified down to the level that is permitted by the <br />available physical characteristics. Early life stages of many species sometimes lack certain <br />anatomical characteristics that allow the specimen to be identified to the genus or species <br />level. In these cases the "lowest practical taxonomic level" may mean only the family level. <br />In instances where proper identification is possible, the Orders Ephemeroptera, Plecoptera, <br />and Trichoptera were identified to genus (and many down to species). Most specimens of <br />other Orders, including Diptera, were identified to the genus level, however, members of the <br />family Chironomidae were only identified to subfamily or tribe. Further identification <br />would require mounting of head capsules - an expensive and time-consuming process. As a <br />means of QA/QC, Dr. Boris Kondratieff (Professor of Entomology at Colorado State <br />University) confirmed identifications in all cases where the identification of a species is <br />difficult or questionable. Population densities and species lists were developed for each <br />sampling site. Data collected will be used in various indices recommended by the Rapid <br />Bioassessment Protocols (Plafkin et al. 1989) to provide information regarding <br />macroinvertebrate community structure, function, and general aquatic conditions. <br />Indices used include Shannon-Weaver diversity and evenness, Family Biotic Index, EPT <br />index, and description of functional feeding groups. Shannon-Weaver diversity and <br />evenness values will be used to detect changes in macroinvertebrate community structure. <br />Draft 2001 Task Summary Report Page 16 <br />Miller Ecological Consultants, Inc. December 18, 2001