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RESULTS AND DISCUSSION <br />My comments on Nonnative fishes in the upper Colorado River Basin and a strategic plan for their <br />control (Tyus and Saunders 1996) are given in Appendix J. Tyus and Saunders (1996) contended that bag <br />limits for riverine population of nonnative sport fish could be liberalized in Colorado's western rivers to <br />facilitate increased angler harvest of predatory species. Furthermore, the Colorado Water Conservation <br />Board passed a resolution in July, 1996 that included a recommendation to remove bag limits on nonnative <br />sport fishes within Critical Habitat. My comments on the removal of bag and possession limits for nonnative <br />sport fish within Critical Habitat in western Colorado are given in Appendix K. During discussions regarding <br />the bag and possession limit removal before the Wildlife Commission, my research project was committed to <br />provide an evaluation of the lifting of bag limits on nonnative warmwater sport fishes and resulting benefits <br />to endangered fishes (Malmsbury 1997). The finalized brochure on nonnative fish control strategies and <br />warmwater fisheries in western Colorado is given in Appendix L. <br />Job No. 5: Native/Nonnative Fish Trophic Economic in Riverine Habitats <br />Job Objective: To estimate trophic impacts of predation by selected nonnative fish fishes and size structures <br />on selected native fishes under varying programs of nonnative fish control and floodplain <br />habitat restoration. <br />Segment Objective 1: No evaluations scheduled under this Job during this segment. <br />INTRODUCTION <br />An approach that will be tested under this Job will be the use of stable isotope analysis (SIA) for <br />food web analysis in the Colorado and Yampa rivers. Stable isotopes represent different atomic weights <br />(neutrons) of elements. The elements C, N, S, H, and O all have more than one isotope. Isotopic <br />composition of natural material can be measured with great precision using an isotope ratio mass <br />spectrometer. The mass spectrometer measures the ratio of light and heavy isotopes in a sample and <br />compares this value to a standard (Peterson and Fry 1987, Lajtha and Michener 1996). <br />7 <br />Stable isotopes are useful in trophic studies of food webs because the isotopes move with little or <br />predictable alteration in food chains (Angradi 1994). Typically, the ratio of 13C/12C is used to identify plant <br />source (habitat-based food dependencies) consumed while the ratio of I INI"N is used to delineate trophic <br />position of an organism or differences in the lengths of food chains (France and Steedman 1996, Michener <br />and Schell 1996). Stable isotope ratios reflect the C and N assimilated by organisms rather than simply what <br />was consumed by an organism (Angradi 1994, Rosenfeld and Roff 1992). Because stable isotopes reflect <br />material assimilated over a period of weeks or months, they may help overcome several shortcomings of <br />traditional food habits investigations such as empty stomachs, regurgitated contents, unidentifiable remains <br />of food items, rapid digestion of key diet components or seasonal shifts in food preference or availability <br />(France and Steedman 1994). <br />The use of stable isotopes to track energy provenance in food webs relies on there being distinct <br />isotopic differences between the sources of material in the environment (France and Steedman 1994, Lajtha <br />and Michener 1996, Peterson and Fry 1987). Recent literature has debated the utility and accuracy of the <br />stable isotope techniques for application in lotic environments since the origins and pathways for assimilating <br />carbon and nitrogen through the foodweb may not be as distinct as in lentic environments (Doucett et al. <br />1996, France 1996). However, this techniques has been applied in the lower Colorado River Basin and was <br />found to distinguish between four bases of aquatic secondary production including upland vegetation, riparian <br />25