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1 <br />"effective distance" is minimized. A. Martinez (CDOV~ has a strong database on many ponds, <br />and some evaluation on the effectiveness of treatments in controlling nonnative fish in ponds. <br />4. Nonnative fish densities in backwaters, pre- and post-regulation periods <br />' Figure 23 shows the sampling locations for three intensive nonnative fish sampling or <br />removal efforts in backwaters of the ISA of the Colorado River from 1997 to 2001. Note that the <br />only dataset that contains information pre-regulation is the Bundy and Bestgen (2001) dataset, <br />which has less spatial coverage than the more recent data sets of Trammell et al. (2002) and <br />Osmundson (2003). This situation posed potential problems in examining trends over the years, <br />but nonetheless, visual comparisons can be made in reaches of the river that have been resampled <br />' in later years. <br />Table 14 shows the percent composition for several nonnative fish species in backwater <br />collections of Bundy and Bestgen (2001), Trammell et al. (2002), and Osmundson (2003) within <br />the ISA. Fathead minnow was clearly the most abundant nonnative present in backwaters at <br />more than 50% over all sampling efforts. Fathead minnows were not caught during the sampling <br />1 efforts of Osmundson (2003) due to the fact that it was an electrofishing effort which favored the <br />capture of medium- and large-sized species. Green sunfish, largemouth bass, black bullhead and <br />white sucker were the next most prevalent species, respectively, based on the samples from these <br />' studies collected by various methods. <br />Relative densities of five nonnative fish species of interest (largemouth bass, green <br />'' sunfish, fathead minnow, bluegill and black crappie) were based on the first-pass sampling <br />efforts of Bundy and Bestgen (2001) and Trammell (2042) and are mapped in Figures 24a-e for <br />1997-2001, and for the pre- (1997-1998) and post-regulation time periods (1999-2001). $ach <br />sampling approach varied in intensity, resulting in large variation in the number of individuals <br />caught for any particular species. In general, these figures indicate no obvious change in the <br />densities of each species over the years, but they do indicate that the highest densities are shifting <br />locations from year to year. This could have been a direct result of removal efforts which may <br />have dampened populations in the subsequent year in particular locations, but these efforts <br />appeared to have had little effect on the overall populations of nonnative fishes in the river. <br />' Further, there appears to have been little change in the composition offish species with respect <br />to native vs. nonnative proportions from 1997-2001 (Figure 25). <br />Proximity analyses <br />1. Comparison of stocking events vs. high backwater densities ("high" densities are locations <br />that fall in the top 10% of density for the particular species being considered) <br />Proximity analyses in the ISA, although expected to potentially reveal patterns not visible <br />to the naked eye, indicated a similar lack of pattern as did the visual analysis. Figure 26 shows <br />that the proximity of high largemouth bass density in backwaters to largemouth bass stockmg <br />locations was not different from a comparison of random backwater locations with low/absent <br />abundance of largemouth bass to the same stocking locations. Fathead minnow also showed no <br />pattern, and as previously mentioned, green sunfish were not stocked. <br />17 <br />