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'~ <br />There were several factors that introduce an unknown level of uncertainly into our study. First, on <br />more than one occasion, nets were run in stages~such that early catch was held in a live car for a <br />period of time before being re-assimilated with the later catch. This allowed an unequal time for <br />digestion or evacuation of GI contents within sub-samples of fish. Potential effects of this <br />protocol on food consumption results are unknown. Next, there were no control samples that <br />could be used to evaluate the effectiveness of the siphoning method vs. surgical extraction of G1 <br />contents. However, studies by others (BioNl/est 1994, Wasowicz and Valdez 1994) suggest that <br />siphoning was nearly 100% effective with roundtail chub Gila robusta and was assumed similarly <br />effective with humpback chub Gila cypha. Bonytail is morphologically similar to these congeners <br />and we are unaware of any reason stomach pumping would be differentially effective among the <br />three species. Finally, an expected linear relationship between fish weight and GI sample size <br />was not observed. Implications of this result are not clear, but if may have been due in part to <br />variation among samples in time elapsed between capture and processing. <br />Acknowledgements <br />Gordon Mueller, U.S. Geological Survey (USGS) contributed substantially to the design and <br />implementation of this project. Jeanette Carpenter (USGS}, Darren Thornbrugh, Robert Colvin <br />and C.O. Minckley (U.S. Fish and Wildlife Service, USFWS), and Michael R. Schwemm (Arizona <br />State University, ASU) provided additional field assistance. Work was performed under ASU <br />Animal Use and Care Protocol No. 05-767R. Appropriate Arizona, California, and USFWS <br />permits authorized collections. U.S. Geological Survey Biological Resources Division provided <br />funding through Cooperative Agreement No. OOCRAG004 Project Award No. 0004CS003 to <br />Arizona State University. Gordon Mueller served as the USGS Project Officer. <br />Literature Cited <br />Bio/West. 1994. Life history and ecology of the humpback chub (Gila cypha) in the Coiorado <br />River, Grand Canyon. Final Report, U.S. Bureau of Reclamation Contract No. O-CS-40-09110. <br />Bio/West, Inc., Logan, Utah. 168 pages + appendices. <br />Clarkson R.W., A.T. Robinson and T.L. Hoffnagle. 1997. Asian tapeworm (Bothriocephalus <br />acheilognath~~ in native fishes from the Little Colorado River, Grand Canyon, Arizona. Great <br />Basin Naturalist 57: 66-69. <br />LaBarbara, M. and C.O. Minckley. 1999. Report on native fish growout facilities at Cibola and <br />Imperial National Wildlife Refuges 1993-1005. U.S. Fish and Wildlife Service, Parker Fishery <br />Resources Office, Parker AZ. 20 pages + tables, figures and appendices. <br />Jonez, A. and R.C. Sumner. 1954. Lakes Mead ad Mohave investigations. Nevada Fish and <br />Game Commission, Carson City. <br />Kirsch. P.H. 1889. Notes on a collection of fishes obtained in the Gila River at Fort Thomas, <br />Arizona, by Lieut. W.L. Carpenter, U.S. Army. Proceedings of the U.S. National Museum 11: <br />555-558. <br />Marsh, P.C. 2000. Fish Population Status and Evaluation in the Cibola High Levee Pond. Final <br />Report, U.S. Bureau of Reclamation Agreement No. 99-FG-30-00051. Arizona State University, <br />Tempe. 11 pages. <br />Mueller, G.A., J. Carpenter, P.C. Marsh and C.O. Minckley. 2003. Cibola High Levee Pond <br />Annual Report 2003. Project Report, U.S. Geological Survey, Fort Collins Science Center, Fort <br />Collins, Colorado. 26 pages. <br />Minckley, W.L. 1973. Fishes of Arizona. Arizona Game and Fish Department, Phoenix. 273 <br />pages. <br />