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After sampling the second backwater, investigators proceeded downstream to the top of <br />the next 5-mile reach before sampling again. Samples were not taken within a 5-mile <br />subreach if no backwaters meeting ISMP's criteria were found. The first backwater sampled <br />in each 5-mile subreach was considered a primary backwater, and the second backwater <br />sampled was considered a secondary backwater. Although there were some differences in <br />how nonendangered fish were handled in primary and secondary backwaters, there was no <br />difference in sampling technique. Therefore, catch rates for YOY Colorado squawfish were <br />calculated using combined data from both primary and secondary backwaters. <br />The relative density, or catch per effort (CPE), of YOY Colorado squawfish was <br />determined by the number collected per area of backwater swept with the seine. Because of <br />the relatively low number of Colorado squawfish collected every year, data are not normally <br />distributed-an assumption required by many. data-analysis techniques. McAda (1989a) <br />investigated several methods of data analysis for the monitoring program and suggested using <br />geometric-mean CPE rather than the more standard calculation of arithmetic mean. The <br />geometric mean is calculated by using a log transformation (log~(CPE+ 1)) before calculating <br />the mean. The mean of the log values is then transformed back to a standard value <br />((2.71828"°)-1). A log transformation improves the normality of CPE data, but <br />unfortunately, low catch rates still result in non-normal data in many instances. Change in <br />CPE over time should reflect change in the number of YOY Colorado squawfish present each <br />fall. <br />Geometric-mean CPE was calculated using each backwater as the basic element rather <br />than each seine haul. The total number of Colorado squawfish collected in both seine hauls <br />in a backwater was divided by the total area seined with- both seine hauls to determine CPE <br />for that backwater. Geometric-mean CPE was then calculated for each monitoring reach and <br />for 10-mile intervals within monitoring reaches. To save space, geometric-mean CPE will <br />be referred to as gmean CPE throughout the remainder of this report. <br />Gmean CPE, calculated by 10-mile subreach, was plotted in histograms to compare <br />general distribution patterns among reaches and years. Gmean CPE for the entire reach was <br />plotted to compare changes over the 11 years of ISMP. <br />4 <br />