Laserfiche WebLink
hauls were taken in the backwater. Effort was recorded as azea swept by the seine. Colorado <br />squawfish were measured, counted, and released alive if they were large enough to be reliably <br />identified. Fish of other species that were also large enough to reliably identify were counted and <br />released. All remaining fish in the first sample were preserved for later identification in the <br />laboratory to check for Colorado squawfish too small to be identified in the field. Non-target fishes <br />were later counted in the laboratory for this sample only. Investigators then proceeded downstream to <br />the next backwater which was also sampled with two non~verlapping seine hauls. Colorado <br />squawfish were measured, counted, and released alive. No other fish from the second backwater <br />were preserved or counted for ISMP. However, a second study evaluating the abundance of <br />introduced species in conjunction with ISMP counts or preserves all specimens collected from all <br />backwaters in the Colorado River. These data will be reported elsewhere. <br />After sampling the second backwater, investigators proceeded downstream to the top of the next <br />5-mile reach before sampling again. Samples were not taken within a 5-mile subreach if no <br />backwaters meeting ISMP's criteria were found. The first backwater sampled in each 5-mile subreach <br />was considered a primary backwater, and the second backwater sampled was considered a secondazy <br />backwater. Although there were some differences in how nonendangered fish were handled in <br />primary and secondary backwaters, there was no difference in sampling technique. Therefore, catch <br />rates for YOY Colorado squawfish were calculated using combined data from both primary and <br />secondary backwaters. <br />The relative density, or catch per effort (CPE), of YOY Colorado squawfish was determined by <br />the number collected per area of backwater swept with the seine. Because of the relatively low <br />number of Colorado squawfish collected every year, data are not normally distributed-an assumption <br />required by many data-analysis techniques. McAda (1989a) investigated several methods of data <br />analysis for the monitoring program and suggested using geometric-mean CPE rather than the more <br />standazd calculation of azithmetic mean. The geometric mean is calculated by using a log <br />transformation (log~(CPE+1)) before calculating the mean. The mean of the log values is then <br />transformed back to a standard value ((2.71828m°°)-1). A log transformation improves the normality <br />of CPE data, but unfortunately, low catch rates still result in non-normal data in some instances. <br />Change in CPE over time should reflect change in the number of YOY Colorado squawfish present <br />each fall. <br />Geometric-mean CPE was calculated using each backwater as the basic element rather than each <br />seine haul. The total number of Colorado squawfish collected in both seine hauls in a backwater was <br />divided by the total area seined with both seine hauls to determine CPE for that backwater. <br />Geometric-mean CPE was then calculated for each monitoring reach and for 10-mile intervals within <br />monitoring reaches. To save space, geometric-mean CPE will be referred to as gmean CPE <br />throughout the remainder of this report. <br />Gmean CPE, calculated by 10-mile subreach, was plotted in histograms to compare general <br />distribution patterns among reaches and years. Gmean CPE for the entire reach was plotted to <br />compare changes over the 8 years of ISMP. <br />Length-frequency distributions were developed by reach for all Colorado squawfish collected. <br />Mean total length and other summary statistics were developed for the four reaches sampled. <br />Number of all species collected was totaled for the first seine haul in primary backwaters. Gmean <br />CPE was calculated for the three most common species in these collections. <br />3 <br />