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Another method of improving statistical reliability would be to reduce the vaziability of ISMP <br />data. Refining habitat preferences of YOY Colorado squawfish and placing further restrictions on <br />locations sampled could reduce the vaziability somewhat. However, certain restrictions aze already <br />contained in the sampling design and analysis of ISMP data did not identify any preferences that had <br />not already been reported (i.e. Haines and Tyus 1990). Studies currently being conducted by UDWR <br />may improve our ability to distinguish habitat preferences of YOY Colorado squawfish. <br />A third method of improving statistical reliability of ISMP would be to incorporate components of <br />mazk and recapture into the sampling design. This option would provide the best data of all the <br />options considered, but it would also be the most labor intensive. Haines and Modde (1993) <br />successfully conducted mazk-recapture population estimates of YOY Colorado squawfish in a short <br />reach of the Green River. However, capturing and mazking the fish required considerably more time <br />than expended with ISMP. It took about 2 days for athree-person crew to collect and mazk fish in a <br />10-mile section of river (B. Haines, Fish and Wildlife Service, personnel communication). ISMP <br />crews aze usually able to sample 30 or more miles a day. Further, a population estimate requires two <br />different recapture efforts which would increase the labor requirements even more. Another factor to <br />consider is increased mortality of the small fish because of increased handling. It is impractical to <br />sample all 390 miles of the four ISMP reaches in this manner. However, mazk recapture could be <br />done in small sections of each reach every yeaz to validate the ISMP data. <br />Consideration should also be given to increasing the amount of information collected on fishes <br />other than Colorado squawfish encountered with ISMP. Evaluation of the entire fish community was <br />a major recommendation by Stanford (1993) in his review of studies in the upper basin designed to <br />develop flow recommendations for the endangered fishes. Although all fishes are counted in about <br />one of four seine hauls now, the data could be improved by identifying and counting all species in a <br />lazger percentage of the seine hauls. Investigators considered this option during a review of ISMP in <br />1989 but felt it was too great of a burden to place on field crews and suggested it be made part of <br />sepaaate studies if reseazchers felt it important (McAda 1989b). Such a separate study is currently <br />being conducted in the Colorado River by the Fish and Wildlife Service. In the second study, CPE <br />calculated for the three most common species (using all seine hauls) generally followed the same <br />trends shown with ISMP (first seine haul in primary backwaters), although there were some <br />exceptions (Fish and Wildlife Service, unpublished data). The less common species were represented <br />in the total catch in about the same ratio as with ISMP data, but there was usually at least one species <br />collected in low numbers that was not observed with ISMP (mean, 2; range, 0-3; Fish and Wildlife <br />Service, unpublished data). Additional work on sympatric fishes in the Green River could also be <br />handled as a related study instead of being added to ISMP. <br />All of the above suggestions should be considered as possible improvements to ISMP. <br />Pazticipants should also consider the relative information gain against the increased effort that some of <br />the options will require. ISMP can undoubtedly be improved. However, the basic sampling design <br />(i.e. seining backwaters in specified reaches with a systematic manner) should remain unchanged. <br />Our ability to monitor long-term trends will be compromised if major components of the sampling <br />program aze modified (Ney 1993). <br />24 <br />