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McBe and Vrijenhoek <br />Conservation Genetics of Desert Fishes 163 <br />GROUPS <br />0.16 <br />0.15 <br />0.13 <br />F- <br />p 0.1 1 <br />O <br />~ 0.10 <br />O <br />~ 0.08 <br />w <br />U <br />z <br />Q <br />0 <br />0.07 <br />0.05 <br />0.03 <br />0.02 <br />0.0 <br />...... <br />....... <br />...... <br />....... <br />....... <br />.. .... .... <br />.... <br />.... <br />.... <br />... <br />.... .... <br />... <br />.... <br />... <br />.... <br />.... ... <br />.... <br />... <br />.... <br />.... <br />... <br />- ......... <br />...... _ <br />......... <br />........ <br />........ <br />......... <br />6 :::_ <br /> D < :> <br /> <br />;:: <br />;. <br /> ';: .:: <br /> F I <br /> <br />....... <br />...... <br />....... <br />.... _ <br />.... . <br />...... ... <br />.... <br />... <br />.... <br />... <br />.... .... <br />.... <br />.... <br />.... <br />.... <br />.... ... <br />.. <br />... <br />.. <br />... <br />.. .... .. <br />....... <br />....... <br />....... <br />.... .. <br />....... <br />.~~~N.~~~~~.~~~~~~~..~~~~~~ <br />......~p ~ ................. <br />.......................... <br />,........., p .......~.~.. <br />.~E. . R , .....~~...~.. <br />............. ........ <br />~Q ~~~ <br />Figure 4. Genetic relationships among Poeciliopsis occidentalis populations The dendogram produced three <br />clusters that correspond with geographical distributions on the map. Apparent nonlinearity of the dendogram <br />scale is due to rounding. (Reprinted with permission from Vrijenhoel~ Douglas; F> Meffe 1985.) <br />3 ). A rapid decline ensued over the last four decades to <br />the point that the fish was included on the Federal En- <br />dangered Species List in 1973. <br />Decline of the topminnow is attributed to widespread <br />habitat destruction and replacement by the introduced <br />mosquitofish (Gambusia affinis) (Meffe et al. 1983; <br />Meffe 1985). In 1983 the topminnow occurred in 15 <br />natural habitats in the United States. Presently, the fish <br />occurs in 12 natural localities in Arizona (Fig. 3), all of <br />which are isolated headwater springs or streams, but it <br />Conservation Biobgy <br />Volume 2, No. 2, June 1988 <br />