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DRAFT <br />559. mm (22 in.) TL (n=120), but ripe females averaged 710 mm (28 in.) TL (n=7) <br />on spawning grounds in the Green River basin. Hatchery-reared. males matured <br />at 5 years and females at 6 years of-age (Hamman 1981). Some of these <br />hatchery fish matured naturally while :others .produced gametes only after <br />injection of pituitary extract (Hamman 1981). <br />Colorado squawfish gonads ripen during spring runoff (May-June). Laboratory <br />and field studies indicate that spawning begins when water temperatures reach <br />about 21oC (70oF) (Hamman 1981; Miller et al. 1982a; Toney 1974; Vanicek and <br />Kramer 1969). Archer et al. (1985), Haynes et al. (1984), and Tyus et al. <br />(1987) found that spawning. occurred between late June and mid-August when <br />water temperature reached 18-25oC (64o-77oF), with peak spawning activity <br />occurring between 22-25oC. Although turbidity has precluded direct <br />observation of spawning behavior, radiotracking and collection data suggest <br />similarities to the northern squawfish (Beamesderfer and Congleton 1981; <br />Patten and Rodman 1969). <br />Radiotelemetry studies and collections of spawning fish have added to the <br />knowledge of Colorado squawfish spawning activities, seasonal movements, and <br />habitat use (Miller et al. 1984; Radant et al. 1983; Tyus and McAda 1984; Tyus <br />et al. 1982b; Tyus et al. 1987; Wick et al. 1983). During the spawning <br />season, adult squawfish have been known to migrate up to 320 km (200 miles), <br />upstream or downstream, to reach spawning areas in the Green River Basin <br />(Miller et al. 1983; Tyus 1985; Tyus et al. 1982a, 1982b). Homing behavior <br />and fidelity to spawning locations has .been indicated for Colorado squawfish <br />in the Green and Yampa rivers (Tyus 1985; Tyus et al. 1987; Wick et al. 1986). <br />Some authors suggest that repeated use of the same spawning areas may reflect <br />a limited availability of spawning habitats rather than true homing (Archer et <br />al. 1985; O'Brien 1984); however, Tyus and Karp (1989) report that migrating <br />Colorado squawfish pass through many miles of potential spawning habitat to <br />reach specific spawning areas in Yampa Canyon. However, not all- adult-sized <br />Colorado squawfish exhibit long-distance migratory behavior associated with <br />spawning, perhaps because they are immature (Tyus and McAda 1984; Wick et al. <br />1983), do not spawn every year (Wick et al. 1983), or spawn near the area in <br />which they reside. <br />Radiotelemetry studies in the Green River basin suggest that spawning is <br />concentrated in two major sites: (1) the lower 32 km (20 miles) of Yampa <br />River canyon; and (2) Gray Canyon of the Green River (Tyus 1985; Tyus and <br />McAda 1984; Tyus et al. 1987;-Wick et al. 1985)-{see Figure 2). Spawning also <br />is suspected in Labyrinth Canyon in the Green River about 50 km (31.25 miles) <br />upstream of the Colorado River confluence (Tyus et al. 1987). Radiotelemetry, <br />collections of ripe fish, and recaptures-have confirmed long-distance <br />migration to these locales, sometimes 100 km (62.5 miles) or more, both <br />upstream and downstream (Tyus et al. 1987; Wick et al. 1983). A total of <br />112 adult squawfish were radiotracked by Service personnel from 1980-85 <br />(Archer et al. 1985; Tyus et al. 1982b, 1987) and five were tracked by <br />National Park Service and Colorado Division of Wildlife workers in 1982 (Wick <br />et al. 1983). Collections made on the two known spawning grounds during <br />1981-85 produced 208 Colorado squawfish, of which 127 were ripe and 160 were <br />in breeding condition <br />8 <br />