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<br />To maintain a genetically healthy hatchery stock that is needed to complete <br />hatchery-related research, it is necessary immediately to obtain: (a) a <br />limited number of wild individuals of the rare fishes as hatchery broodstock; <br />or (b) milt from wild males which would be used to fertilize eggs of female <br />broodstock in the hatchery. The latter method is preferable, since wild <br />individuals should remain in their natural environment. Genetic studies may <br />be needed on all species to test for variation among wild individuals from the <br />different subbasins. <br />Refugia for all four species should be maintained in more than one hatchery as <br />a safeguard against disease and possible extinction. The bonytail may benefit <br />the most from a hatchery refugium, since it is presently on the verge of <br />extinction in the upper basin. Because the immediate goal is to prevent <br />extinction of the bonytail, major emphasis should be placed upon stocking of <br />bonytails in conjunction with a continuing habitat analysis and major <br />population monitoring and research effort. <br />4.3.4 Hatchery Production Program <br />If the research program confirms that hatchery-reared fish will survive and <br />reproduce successfully in the wild, then a hatchery production program should <br />be implemented. At that time, additional hatchery facilities may be needed to <br />produce individuals for reintroduction into the wild (see Section 5.4 Capital <br />Funds). If introductions prove successful, there is a possibility that <br />hatchery-reared individuals could augment existing populations, inhabit <br />historic habitat, or expand the present range of these species. <br />4.3.5 Selected Course of Action <br />a. The Service will use existing facilities and capabilities at Dexter (New <br />Mexico) and Willow Beach (Arizona) National Fish Hatcheries or other <br />facilities (if necessary) to raise the target number of rare fishes needed <br />for the research program, unless or until grow-out ponds can meet these <br />needs. Existing hatchery facilities must be capable of rearing disease- <br />free, genetically viable and healthy fish eggs, larvae, and juveniles. <br />b. Hatchery broodstocks for the bonytail chub, the Colorado squawfish, or the <br />razorback sucker must be enlarged by capture of wild individuals or <br />preferably by introduction of wild gametes (eggs and milt) into the <br />broodstocks for upper basin fish. A broodstock for the humpback chub will <br />also be developed. Two or more refugia for each species are recommended. <br />c. Procedures for producing the rare species in hatcheries for the research <br />program will be developed by the Service. This will include details on: <br />1. Maintenance of genetic diversity; <br />2. Collection and transport of gametes or adults from the wild to the <br />hatchery facility; <br />3. Procedures for spawning at the hatchery; <br />4. Location of fish stocks; <br />5. Details on research projects; and <br />6. Method of transport and release to the wild. <br />4-16 <br />J <br /> <br /> <br /> <br />f <br /> <br /> <br />1 <br />1 <br /> <br />1 <br />11 <br /> <br /> <br /> <br /> <br />n <br />