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Box 7. Guidelines for preparation of a stocking plan for release of captive <br />-reared endangered and candidate fishes. <br />1. Define objectives for stocking fish and relationship between <br />these objectives and the Recovery Plan. <br />2. Identify alternative management options that could achieve the same <br />objectives and discuss why stocking was selected over these options. <br />3. Provide justification of stock selected for the stocking effort <br />(i.e., broodstock developed from a wild stock inhabiting the river <br />reach to be stocked or nearest neighbor). <br />4. Provide the criteria (e.g., geographic distribution and abundance, <br />identified spawning sites, migrations, known movement and interchange <br />between stocks, and data from genetic surveys) used for identification <br />and characterization of the captive fish stock to be used in the <br />effort. <br />5. Describe the status and trends of the target fish stock including <br />biological, demographic, and environmental data for that specific <br />stock. <br />5. Describe the breeding strategy used to maintain genetic diversity in <br />the captive-reared fish. <br />7. Describe the stocking location including the available habitat for <br />each life stage of the fish species. <br />8. Provide rationale for the size of fish to be stocked and the mark or <br />tag used to identify stocked fish if recaptured. <br />9. Provide rationale for the numbers of fish to be stocked. <br />10. Describe the timing of stocking, including frequency if repeated <br />stocking is planned. <br />11. Discuss the likelihood of success as well as genetic risks to the <br />target stock and risks to other endemic fish species in the <br />ecosystem. <br />12. Describe methods for systematic monitoring, evaluation, and reporting <br />of the stocking effort. <br />fish from pedigreed family lots at the time of release will provide genetic diversity <br />that will maximize effective population size and reduce the potential for inbreeding <br />while enhancing the potential for adaptation and survival after stocking (Allendorf <br />1993: Kapuscinski et al. 1993). Captive rearing results in a gain in total offspring <br />but there may be a simultaneous reduction in the effective population size (Ryman and <br />Laikre 1991). A reduction in effective population size will be accompanied by <br />excessive loss of genetic heterozygosity. This is especially true if the absolute size <br />of the wild population is small. Ryman and Laikre (1991) strongly recommend that the <br />risk of losing genetic variability through captive breeding should be carefully <br />19