9411

• UCREFRP Catalog Number: 9411
• Author: Williamson, J. H., D. C. Morizot and G. J. Carmichael.
• Title: Biochemical Genetics of Endangered Colorado Pikeminoow from the Green, Yampa, Colorado, and San Juan Rivers.
• USFW Year: 1998.
• USFW - Doc Type: \
• Copyright Material: NO

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Table 2. Summary of scores for individuals from four populations using thyroxin-like PCR
product digested with ten restriction enzymes. No polymorphism was detected.
THYROXIN-LIKE LOCUS (1100 bp)
Restriction Recognition Fragment Sizes Number of Individuals Scored
Enzyme Sequence Dexter 174 Dexter 181 Yampa Green
Bbs I GAAGAC(2/6) 275, 325, 500 7 7 9 7
Dde I CTNAG ?, 100, 350, 600 7 7 9 7
Hae III GGCC 250, 850 7 7 9 7
Hha I GCGC 170, 440, 490 7 7 9 7
Hind III AAGCTT 5301 570 7 7 9 7
Hinf I GANTC ?, 160, 190, 250,
350 7 7 9 7
Msp I CCGG 100, 300, 700 7 7 9 7
Nla III CATG ?, 145, 155, 170,
450 7 7 9 7
Rsa I GTAC ?, 150, 800 7 7 9 7
Tfi I GA(A/T)TC 250, 850 7 7 9 7

?: very small, indistinct bands of unresolved sizes are produced by the two restriction
enzymes containing "N" (redundancies) in their recognition sequences.
a
b
a
a
N