9411

• UCREFRP Catalog Number: 9411
• Author: Williamson, J. H., D. C. Morizot and G. J. Carmichael.
• Title: Biochemical Genetics of Endangered Colorado Pikeminoow from the Green, Yampa, Colorado, and San Juan Rivers.
• USFW Year: 1998.
• USFW - Doc Type: \
• Copyright Material: NO

1
1
1

1

1
w

1

3
Table of Contents
Page Number
Title Page ........................................................ ..................................................... 01
Acknowledgments ............................................................................................................. 02
Table of Contents ........................................................ ..................................................... 03
List of Tables ........................................................ ..................................................... 03
List of Figures ........................................................ ..................................................... 03
List of Key Words ........................................................ ..................................................... 03
Executive Summary ............................................................................................................. 04
Report Contents
Introduction ............................................................................................................. 06
Methods ............................................................................................................. 07
Biochemical genetic analyses 08
Nuclear DNA analyses 08
Findings and Discussion ........................... ....................................................... 09
Biochemical genetic analyses 10
Allozyme variability in wild adults 10
Allozyme variability in juveniles 11
Allozyme variability in Young-of-the-Year 11
Genetic variability in hatchery populations 11
Genetic identification using nuclear DNA 11
Conclusions ....................................................... ...................................................... 12
Allozyme variability in wild adults 12
Allozyme variability in juveniles 12
Allozyme variability in Young-of-the-Year 12
Genetic variability in hatchery populations 12
Implications of allele frequency estimates 12
Implications for genetic management 13
Recommendations ......................................... ....................................................... 13
Bibliography ......................................... ....................................................... 15
30
Appendices ................................................................................................
List of Tables.
Table 1.- List of protein-coding loci resolved in Colorado pikeminnow, alphabetized by gene symbol. (17)
Table 2.-Protein-coding loci resolved in various Colorado pikeminnow tissues, alphabetized by gene symbol. (19)
Table 3.- Allele frequencies of polymorphic protein-coding loci resolved in adult Colorado pikeminnow
populations collected from four rivers. (22)
Table 4.- Allele frequencies of polymorphic protein-coding loci resolved in juvenile Colorado pikeminnow
populations collected from 3 locations in the Colorado River. (23)
Table 5.- Allele frequencies of polymorphic protein-coding loci resolved in YOY Colorado pikeminnow
populations. (24)
Table 6.-- Allele frequencies of polymorphic protein-coding loci resolved in combined Green River and Colorado
River YOY Colorado pikeminnow populations from this study and that of Ammerman and Morizot
(1989XA&M). (25)
Table 7.-- Allele frequencies of polymorphic protein-coding loci resolved in Dexter National Fish Hatchery and
Technology Center broodfish populations and four combined wild populations (Yampa River, Green
River, Colorado River, and San Juan River)Colorado pikeminnow populations. (27)
List of Figures.
Figure I.- Colorado pikeminnow sensitive areas identified by the upper Colorado River Coordinating Committee
(1987). (28)
Figure 2.-- Cluster analysis of six Colorado pikeminnow populations for 12 loci. (29)
List of Key Words.
Colorado pikeminnow, Ptvchocheilus lucin allozymes, polymorphisms, biochemical genetics, nuclear DNA.
1