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1 1 ?I 1 1 I 1 Sixty adult bonytail were transferred from Dexter National Fish Hatchery NM to a backup refuge at Horsethief State Wildlife Area near Grand Junction CO in 1993. However, most of these fish died from the parasite Ichthyophthirius multifilis; only one fish survived. Twenty-five paired matings (n = 50) will be made of Lake Mohave bonytails at Dexter National Fish Hatchery in New Mexico during the spring of 1994. Fertilized eggs will be sent to the Valley City National Fish Hatchery, North Dakota, and will be reared in circular fiberglass tanks using heated water for the first year of life. These fish will be PIT-tagged when they are large enough and transferred to refuge ponds at the Horsethief State Wildlife Area CO to be reared as broodstock for restoration stocking in the upper basin. It has been demonstrated that the bonytail can readily hybridize with other Gila (Hamman 1981b). Therefore, stocking in the Upper Colorado River Basin will not be done until the results of Gila genetics and systematics studies are completed and evaluated. An interim report on the Gila work is scheduled for 1995 and a final report for 1997. The Utah Division of Wildlife Resources has the responsibility to develop a restoration stocking plan for the Green River during 1994-1995. Specific Recommendation 6. The Program should adopt a policy to place all endangered fish stocks in jeopardy of extinction or valuable experimental fish in two refuges (primary and backup) for safe keeping. This recommendation was supported by the participants at a work group meeting on September 1, 1992 and at other Biology Committee meetings subsequent to that date. 3. Humpback chub. Collect 30 Canyon in 1995 to be placed in Area CO. The reason for colle, that Program participants will Gila species in 1994 that will captivity. adult humpback chubs from Blackrocks a refuge at the Horsethief State Wildlife sting the fish in 1995 instead of 1994 is gain experience in culture and handling of be helpful in maintaining this species in 4. Colorado sauawfish. Continue rearing Colorado squawfish from the Upper Colorado River presumptive stock at the Horsethief State Wildlife Area CO and maintain fish from this stock at a backup refuge in Dexter National Fish Hatchery NM. Seventeen pedigreed family lots of this stock were developed from spawning adult Upper Colorado River squawfish at the Dexter National Fish Hatchery and Fish Technology Center NM in 1991. Fertilized eggs from these fish were reared at the Experimental Fish Hatchery, Bellvue CO during their first year of life. They were PIT- tagged, divided into three groups, and maintained in refuges at the Horsethief State Wildlife Area CO, Dexter National Fish Hatchery and Fish Technology Center NM, and Wray State Fish Hatchery CO as broodstock. The squawfish at Wray will be transferred to the Horsethief and Dexter facilities as primary and secondary refuges, respectively, early in 1994 and maintained until they are needed for experimental stocking in Program-approved field studies. The remaining stocks of squawfish were assigned priority "2". However, it should be emphasized that the 39