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f <br />The importance of maintaining detailed and accurate propagation records <br />for captive broodstocks cannot be overemphasized. Records should include <br />the origin, genetic profile, and unique identifying number of each adult <br />broodfish as well as its production record and progeny performance. <br />C. Estimating the Number of Fish Required to Produce Broodstocks. The <br />number of endangered fish needed at the end of each growing season to <br />produce broodstocks is dependent upon the attrition (i.e., mortality) <br />rates of the fish at the end of each growing season. Conservative <br />attrition rates were estimated by consensus among members of the <br />Propagation Subcommittee and Biology Committee. These attrition rates <br />will be refined from empirical data from captive propagation of the fish <br />in different facilities. Estimates of razorback suckers or Colorado <br />squawfish needed at the end of each growing season until the fish mature <br />and can be maintained as broodstocks in a primary and a backup refuge are <br />provided in Figure 3. To be certain that about 30 adults (preferably <br />equal sexes) from each family lot are available as broodstocks in primary <br />and backup refuges, the numbers of fish required at the end of each <br />growing season is estimated to be: 1 - 500 fish; 2 - 250 fish; 3 - 200 <br />fish; 4 - 160 fish; 5 - 152 fish; and 6 - 144 fish. <br />As experience is gained in effective culture of the endangered Colorado <br />River fishes, the numbers of fish required to develop broodstocks may be <br />reduced and would require fewer resources (i.e., space for propagation, <br />personnel, funding, etc.). Therefore, a scenario involving fewer fish <br />per family lot as broodstocks may be selected depending upon the <br />objectives of the stocking plan. Since mortality of fish during the <br />first year of culture may be highly variable, the focus should be on <br />estimating the number of adult fish that can reasonably be expected to <br />survive from the number of fish at the end of the first growing season <br />from the following scenarios: <br />Age 1/ Num er o endangered is at the en <br />of each growing season 2/ <br />Fert. Egg 5,000 4,000 3,000 2,000 1,000 <br />' 1 500 400 300 200 100 <br />2 250 200 150 100 50 <br />3 200 160 120 80 40 <br />4 160 128 96 64 32 <br />5 152 122 91 61 30 <br />6 144 116 87 58 28 <br />1/ The age refers to the end of each growing season. <br />2/ Estimated attrition rates are: fertilized eggs 90%; between 1st <br />and 2nd growing season - 50%; between 2nd and 3rd - 20%; between <br />3rd and 4th - 20%; after 4th - 5%. <br />The Gila species will mature after four growing seasons when they <br />are about 14 inches in total length based on estimates of growth <br />for the Upper Colorado River Basin. It is entirely possible that <br />growth will be much better in ponds and the fish would reach a <br />29