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area of growout ponds has not been determined because stocking <br />plans have not been developed for suitable habitat in the upper <br />basin. <br />VII. Permanent Propagation Facilities in the Upper Basin <br />The Program will have permanent propagation facilities at four sites <br />by September, 1996: <br />1. Craig Municipal Facilities, Craig, Colorado <br />(Backup Refuge for Green River Subbasin) <br />2. Horsethief State Wildlife Area, Grand Junction, Colorado <br />(Primary Refuge for Upper Colorado River Subbasin) <br />3. Ouray Endangered Fish Facility, south of Vernal, Utah, and <br />(Primary Refuge for Green River Subbasin) <br />4. Wahweap State Fish Hatchery, Big Water, Utah <br />(Backup Refuge for Upper Colorado River Subbasin and <br />Production) <br />VIII. Current Propagation Efforts in the Upper Basin <br />A. Razorback sucker - Middle Green River Stock. Wild adult razorback <br />suckers (Middle Green River stock) are being maintained at the <br />Ouray Endangered Fish Facility. Nineteen wild adult razorbacks <br />from the Middle Green River stock are in captivity at the Ouray <br />Endangered Fish Facility. Thirty-one additional wild fish are <br />needed to develop 25 pedigreed family lots of this stock. <br />Currently, four family lots from paired coatings are being <br />maintained as broodstock. <br />Additional wild razorback suckers will be collected from the Middle <br />Green River and Upper Colorado River for continued broodstock <br />development during the spring of 1996. Adult fish that are <br />presently in captivity will be spawned to continue development of <br />the broodstock until 25 pedigreed family lots are produced <br />(Williamson and Wydoski 1994). <br />During 1996, ten wild adult razorback suckers will be collected <br />from the Middle Green River prior to or after the spawning season. <br />During 1996, some wild adults that were used to develop pedigreed <br />family lots will be released into the Middle Green River near the <br />River Mile where they were collected (if known) of in the river <br />reach through the Ouray National Wildlife Refuge. If the stress of <br />capture, transport, and handling inhibits maturation in fish <br />collected prior to the spawning season, these fish will be held and <br />spawned in 1997. Razorback suckers will not be disturbed during <br />the 1996 spawning season on Razorback/Jensen Bar. <br />A feasibility study of DNA fingerprinting to determine the <br />relatedness of captive-reared razorback suckers will be completed <br />shortly (target date of March 31, 1996). If this genetic technique <br />provides the tool to ensure that family lots are unrelated, adults <br />5