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<br />During 1994 and 1996 zooplankton was more abundant for a fourto six week period, whereas in <br />1995, zooplankton remained over 20/1 zooplankters for nearly two months. In 1995 and 1996, <br />zooplankton numbers peaked after the wetland was disconnected (396 m3/s) from the river. <br /> <br />Chapter Two: <br />Fish Abundance and Composition <br /> <br />Methods <br />Gear used to capture fish differed each year of the study in an effort to increase efficiency <br />of sampling. Differences in gear used prevented annual comparison of catch per unit effort. One <br />consistency through the study was an attempt to sample fish at one week intervals after the <br />wetland was connected to the river, and two week intervals after the wetland was separated from <br />the river. Fish collections in 1994 began 6 June and ended 5 October; in 1995 sampling began <br />25 May and ended 31 August; and 1996 samples were collected between 15 May and 9 July. In <br />1994 seine collections did not begin until 29 June because of the difficulty in finding seineable <br />locations. Effort also was different among years. During 1994 each weekly effort consisted of <br />one experimental (13 mm x 25 mm x 38 mm bar mesh) gillnet 46 m long and between 9 and 12 <br />seine (4 m long and 3.2 mm mesh) hauls per week (see Appendices 1,2, and 3 for sampling <br />effort per year). On and after 28 July, 1994, a second 55 m long, 38 mm bar mesh gill net was <br />added to the sampling effort. Gillnets were set over night and both gillnet and shoreline seine <br />sampling locations were selected arbitrarily. <br />In 1995 and 1996 specific sampling designs were followed. A grid series was overlaid on <br />a map of Old Charley Wash and sampling sites were selected randomly. Sampling effort in 1995 <br />consisted of three fyke nets (.9 m tall and 1.5 wide horizontal first hoop dimension, and 13 mm <br />mesh) and four 23 m experimental (13 mm x 19 mm x 25 mm bar mesh and 152 mm, 178 mm, <br />and 203 mm panels) trammel net sets, four circular seine collections, four cylindrical minnow <br />traps (3.2 mm mesh), and three light traps per week. Cylindrical minnow traps were .4 m long <br />and .2 m in diameter. Circular seine hauls consisted of placing a 22.9 m bag seine (3.2 mm <br />mesh) in a circle, pulling out the vegetation from the circle, constricting the seine, and retrieving <br />the fish from the bag of the seine. This procedure was modeled after Bayley and Herendeen <br />(1994). Fyke net and minnow trap sampling locations were selected randomly only from <br />shoreline grids and trammel nets and light traps were selected randomly only from open water <br />grids. Concern for mortality on adult razorback sucker caused us to set trammel nets for only <br />two hours in the morning. Nets were checked midway through each set to remove any <br />endangered fishes that might be caught. Following capture, fish were identified to species, and <br />length (Total length in mm) and weight (to the nearest gr) recorded. <br />In 1996, fish were sampled with smaller mesh fyke nets (6 mm mesh), trammel nets <br />(same as used in 1995), tall (.9 m) clover-shaped minnow traps (3 mm mesh), short (.5 m) <br />clover-shaped minnow traps (3 mm mesh), and light traps. Clover-shaped minnow traps <br />consisted of three circular cylinders, 0.48 m in diameter, that were connected with an aperture of <br />3.2 mm. During 1996, sampling sites were determined the same way as in 1995 (i.e. randomly <br />selected grids) with fyke nets, short clover-shaped minnow traps and light traps set in only <br />randomly selected shoreline grids. Trammel nets and tall clover-shaped minnow traps were set <br />only in randomly selected off-shore grids. Each weekly sampling effort consisted of eight fyke <br />nets, eight tall clover-shaped minnow traps and eight short clover-shaped minnow trap sets, four <br /> <br />17 <br />