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<br />portion of fish could be released into eddies and backchannels of the mainstem, with <br />equal numbers of fish released above and below the LCR. Mark-recapture efforts might <br />reveal which is the optimal strategy. Another option may be to stock fish in the left hand <br />channel of the LCR at the confluence region. This is a fairly large pool (probably> 2 <br />acres); generally with very slow currents and some shoreline ledges and vegetation for <br />cover. Here, it may be possible to re-acquaint the fish to LCR waters without potentially <br />overburdening the LCR. From here, the fish would have limited access to the LCR, or <br />full access to the mainstem Colorado River. An important factor determining release <br />sites should be the ability (or lack thereof) to set up soft-release protocols (i.e., short- <br />term holding pens). <br /> <br />Efforts should be made to avoid releasing fish under harsh environmental extremes. <br />For example, fish should not be released under flooding conditions in the LCR (although <br />mild-flowing turbid conditions may be acceptable). Likewise, fish should probably be <br />released in the mainstem under periods of minimal fluctuating flows, or periodS of low <br />flows with decreased velocities. To do otherwise may invite undue mortality. An <br />adaptive approach should be taken (i.e., release times should be flexible and dictated <br />by riverine conditions and flows, rather than by inflexible schedules). Paying attention <br />to current and expected hydrographs, both in the LCR and in the mainstem will be <br />important. <br /> <br />In summary, because of concerns about potential unanticipated secondary effects <br />related to carrying capacity, density dependence, and disease, and in an effort to <br />increase recruitment to the main LCR population, it is suggested that the first attempts <br />to release supplemental stocked fish are done in the mainstem Colorado River between <br />river mile 58.8 and 64.5 (i.e., in the backchannels and eddies between Kwagunt and <br />Lava/Chuar Rapids). Should these attempts prove unproductive, less conservative <br />measures may need to be taken (i.e., release near the confluence region, or higher up <br />in the LCR watershed). <br /> <br />Where could the supplemental fish be grown? <br /> <br />It is not the purpose of this document to specifically identify a hatchery where <br />supplemental fish could be grown. Attributes that a facility should possess include all <br />those listed under the previous section for captive brood stock (e.g., experienced <br />personnel, committed long term funding, ability to keep fish unquestionably isolated <br />from other Gila spp., appropriate water flow and temperatures, quarantine facility, etc.). <br />Raceways, circulating tanks, or even outdoor ponds may be useful for grow-out of age-O <br />fish, and the process should be adaptive in nature. <br /> <br />It is strongly recommended that the facility have the capabilities for naturalistic rearing <br />(e.g., exposing fish to moving water currents, natural substrates, cover types, periodic <br />elevated levels of turbidity, etc.). For example, marl, sands, gravels and substrate rocks <br />could be obtained from the LCR. This could be accomplished by transporting them via <br />boat. In addition, natural food types and some exposure to predators would be <br />desirable (see Brown and Laland 2001 for review). <br /> <br />33 <br />