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<br />different sampling dates using strategies one or two. <br /> <br /> <br />Disadvantage: Like the previous strategy,.this strategy does not allow for the detennination <br /> <br />of the variability among similar habitats within the same reach. Another <br /> <br /> <br />disadvantage of this strategy is that it assumes a representative location of <br /> <br />each habitat exists and that it was selected. <br /> <br /> <br />Recommendation: I recommend carrying out strategy one on the initial sampling trip and <br /> <br />strategy two on subsequent trips. Strategy one will give us an understanding <br /> <br />of the variability in invertebrate communities among similar habitat types <br /> <br /> <br />within the same reach and allow to calculate appropriate sample sizes for <br /> <br />each habitat type. Use of strategy two after this initial trip will save money <br /> <br />and still provide substantial information on the variability in invertebrate <br /> <br /> <br />assemblages among habitats and among reaches over time. <br /> <br />Thus, during the initial sampling trip a subset of sampling locations of each habitat type within <br />each reach would be sampled. All samples collected from the same habitat type within the same <br />reach would be kept separate. This procedure would be repeated for each habitat type present in <br />each reach. Within lengthy reaches sampling sites should be selected such that samples are <br />collected from upper, mid, and lower sections of each reach. On subsequent trips, samples from <br />like habitats within the same strata would be coinbined. The same locations need not be sampled <br />on subsequent sampling trips. A summary of the number of samples to be collected is shown in <br />Table 3. <br /> <br />6 <br />