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Colorado Ui~lch Ii'etlanc~s Beach Test <br />Tech»~cal ~1len~oranc~rin~ <br />2.0 Methods <br />The followilig section desci7bes the methods associated with cell consUuction, substrate recipe <br />design, floe- through cells and «ater quality, daily refilling of the Colorado Gulch head tank, and <br />weeldy collection of «°ater quality aliquots for laboratoi-~- analysis. The bench scale test will be <br />housed in the CMC NRM Atrium (Greenhouse) in order to provide a stable environment for the 8 <br />~~ eek period. Supportilig information provided bar J. Gusek is provided in Appendix A to this <br />document. <br />2.1 Bioreactot~ Cell C'onstfzrctiorr: Each cell will be constructed using a plastic 30-gallon tank, ~~ith a <br />detachable lid. A 1/2" drainage hole will be drilled at the bottom of the can. A 10-Slot <br />pohwinylchloride (PVC) French drain, 2" outer diameter, 1 3/4" inner diameter ~~-ill be connected to a <br />male coupling with a gasket, and placed through the hole inside of the can. The plumbing scheme is <br />sho«m bolo«- in Figure A. The drain tube will then be firmed in an upright position. The bottom 5-6" <br />of each cell will be filled ~~ ith drinkilig ~~ ater grade # 10-#20 silica sand and covered ~yith a nylon <br />netting. The silica sand will hold the French drain in place and along with the nylon netting will help <br />filter and aid drainage. Figure 2 shows a general schematic dra~~ ing of a bioreactor cell. <br />cage Hose <br />Coupling <br />French Dravi Crasket on inside and outside <br />Figure 2 <br />2.2 ~S'ubst~ate Recife Design: James Gusek provided the designs of the four substrate recipes tested; <br />each recipe ~~as designed to target specific metals removal processes. These substrate recipes were <br />used in a similar bioreactor bench scale test run by Karl Ford (BLM) at the Dinero Tumiel. A list of <br />materials, provided by Jim Gusek, included the required substrate quantities of alfalfa hay, cow <br />manure, limestone, «-ood chips, sa~~-dust, and limestone kiln dust. A Control Cell is also included that <br />has a composition equivalent to the average substrate composition of Cells 1-4. CMC NRM acquired <br />these materials from local businesses and contributors for minimal costs. <br />Each cell consisted of different combinations of the acquired materials iii an attempt to determine the <br />most effective metal removal recipe. The cell recipes are sho«7i in Table 1. <br />7 CMC NRM <br />