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suggest that the substrate material is contributing additional manganese to the effluent <br />water. It is unlalown which substrate material may contain manganese. <br />COIICIUSlo11 <br />The cells that received the Colorado Gulch influent water showed a definite ability to <br />remove aluminum, copper, and zinc for which all substrate mixtures would be adequate <br />for a large scale SRB system for AMD water in Colorado Gulch. However, since the test <br />was completed during the fall/early-winter months, the contributions of AMD from the <br />Little Frying Pan Gulch and Tiger Mine Complex is likely small compared to spring run- <br />off. <br />The elevated levels of iron and manganese are likely to originate from the substrate <br />material since the concentrations in Colorado Gulch and municipal drinking water had <br />lower levels than the effluent from each cell It would be beneficial to digest each <br />substrate material to determine where the source of excess iron and manganese are <br />originating. <br />6 <br />