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<br />J <br />J <br />t <br />t <br />t <br />J <br />I <br />I <br />J <br />I <br />I <br />I <br />J <br />, <br />J <br />J <br />t <br />, <br />1 <br /> <br />~~lljljo <br /> <br />Procedures <br /> <br />Twenty-six fish were collected from five sites on the San Juan River between Farmington, <br />New Mexico, and the Colorado. Utah border (see figure 1) using rafts equipped with <br />gasoline generator-powered electroshocking equipment. The fish samples were dissected <br />using stainless steel instruments, and the bile extracted directly from the gall bladder with <br />sterile needles and evacuated blood specimen tubes. Individual fish were examined in the <br />field for occurrences of external abnormalities. Bile samples were frozen and sent to the <br />Geochemical and Environmental Research Group at Texas A & M University (GERG), an <br />analytical laboratory under contract with the USFWS. <br /> <br />The samples were tested by GERG for the individual PAH metabolites, naphthalene, <br />phenanthrene and benzo[a]pyrene, uSing high performance liquid chromatography (HPLC) <br />with florescence detection. In this method, bile is injected directly onto an HPLC system <br />and specific florescence is recorded at appropriate wavelengths for each of the three <br />metabolites. Results were calculated in ng/g wet weight and can be found in Table 2 and <br />in raw form in Appendix A. <br /> <br />4 <br />