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<br />Arizona Game and Fish Department <br />Biological Evaluation: Kanab Ambersnail <br /> <br />July 1998 <br />Page 9 <br /> <br />Table 1. Reduction of KAS source population as a result of <br /> relocating immature individuals in August 1998*. <br />Number of immature KAS Percent reduction in the <br />relocated (,;5 mm) source population <br /> 150 0.5 % <br /> 250 0.8 % <br /> 500 1.7 % <br /> <br />. Total population size estimate of 29,842 in August 1998 is based on 1995 (avq. <br />of June and Sept.), 1996 (avq. of July and Sept.), 1997 (August only) data (IKAMT <br />1998) . <br /> <br />relocation purposes will collected from the lower zone of <br />vegetation. A smaller proportion (ca. 25%) of KASs will be <br />collected from the middle and upper zones to ensure a genetically <br />variable founder pool. <br /> <br />KASs will be removed from host vegetation at VP using <br />entomological-grade forceps and/or soft, camel-hair brushes and <br />petri dishes. KASs will immediately be placed into sealable, clear <br />plastic containers (2L volume). Identification of KASs and other <br />VP mollusks (Catinella, Physa=Physella, and Fossaria) will be <br />verified by illustrations or macrophotos of shell morphology on the <br />outside of each container. A small amount of primary vegetation <br />and damp litter will also be placed in the container as shelter and <br />a food source for collected KASs. Saturated paper towels will line <br />the bottom of each container to help maintain moisture and provide <br />cushion for transport. Small holes (0.5 mm diameter) in the lids <br />of the containers will provide ventilation during translocation. <br />Each container will hold a maximum of 50 individuals. Containers <br />will be labeled with the date, number of KASs, type of host <br />vegetation, patch location and collector. At the end of the <br />collection day, J. Sorensen (AGFD) will inspect each container as <br />a quality control measure. <br /> <br />A thermometer will be attached to the inside of one transport <br />container (per group) to monitor temperature variation during <br />transit. Prior handling experience has shown that immature <br />ambersnails in enclosed containers can tolerate temperatures <br />between ZOC and 25'C without mortality (pers. obs., J. Sorensen, <br />AGFD). Transport containers will be stored in a large ice chest <br />while in transit to establisp~ent sites. A layer of block ice and <br />damp packtowels or burlap will line the bottom of the ice chest to <br />