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<br />7 <br /> <br />and elsewhere in the basin. Evolutionary Significant Units (ESU) can then be identified, and <br />findings compared with unpublished data from other Reclamation-funded studies (i.e., Gila <br />Taxonomy Project). Genetic variation will also be analyzed on a finer scale within- and <br />among-populations by evaluating faster evolving DNA regions, including 15 microsatellite <br />loci and at least one intron region. Genetic structure will be assessed among G. cypha <br />populations, and levels of gene flow will be identified. This approach will determine if <br />distinct Management Units (MUs) are present either within GC or the entire Colorado River <br />basin, and if indeed this is a factor to be considered in recovelY efforts. By contrasting <br />results from different molecular markers, recent or historic population events can be <br />inferred. Further, estimates for population parameters such as N, (effective population size), <br />and Nm (number of migrants) can be explored. Assessment of genetic relationships and <br />estimates of population parameters based on molecular data will produce a solid basis from <br />which to derive recommendations for adaptive management of G. cypha within GC. <br /> <br />V. Study area: Colorado River Basin (known HBC distribution) <br /> <br />VI. Study Methods/Approach: <br /> <br />For a statistically sound analysis, a sample size of50-100 individuals/population will be <br />needed. In this regard, the PIs have already compiled numerous tissue samples during the <br />past five years of research on G. cypha populations. The following have been extracted and <br />21 I of these evaluated for sequence variation: 214 MCR individuals, 200 from Little <br />Colorado River (LCR), and 113 from Upper Basin populations. However, additional <br />samples are needed to meet project objectives. Based upon their GC experiences, the PIs <br />suggest these can best be obtained from research trips that maximize fishing effort at <br />particular MCR areas. Sampling efforts must therefore be site-specific and must require trips <br />in addition to those already scheduled for standard GCMRC monitoring efforts. Analytical <br />procedures will be placed in a deductive framework by using rigorous statistical procedures <br />to test hypotheses arranged within a hierarchical setting. Considerable efforts have already <br />been undertaken by the PIs, not only with regard to familiarity with sample sites within GC <br />and the compilation of samples, but also through establislunent of methodologies, <br />techniques, requisite permits and collaborations. <br /> <br />VII. Task Description and Schedule: <br /> <br />This project was implemented in FY02 and will be completed in FY04 <br /> <br />VIII. FY-2002 Work <br />Deliverables/Due Dates: Quaterly Reports- <br />Budget: $52,491 <br /> <br />Created on 5/6/2003 5:41 PM <br />